Illumina NextGen Sequencing
DNAVision was one of the first sequencing service provider using the breakthrough Illumina sequencing technology.
Several sequencing options have been developed and are available:
Throughput: Up to 120 gigabases per run (400 million reads)
Multiplexing: Ability to analyze a large number of libraries in a single run
Read size: Up to 2x 300 bp
Paired-ends: Sequencing inserts using forward and reverse primers
Illumina MiSeq and Illumina NextSeq sequencing machines are available on site.
DNAVision is the leading DNA analysis company providing highest quality grade Sanger sequencing. DNAVision is one of the first laboratory in the European Union to be ISO 17025 certified for Sanger sequencing (SOP-SEQ-001).
DNAVision develops and validates sequencing projects in order to find, for example, new mutations in a gene of interest. In-house sequence analyses are used as the "gold standard" for the validation of genotyping assays.
Sanger Sequencing is one of the most robust technique for clinical genotyping of SNP.
TaqMan Real-Time PCR Assays
In combinaison with Sanger Sequencing, SNP genotyping using TaqMan assays is widely used in clinical projects for its robustness and accuracy.
Each TaqMan SNP Genotyping Assay includes two allele-specific TaqMan MGB probes containing distinct fluorescent dyes to detect specific SNP targets.