Technologies
Illumina ​NextGen Sequencing
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DNAVision was one of the first sequencing service provider using the breakthrough Illumina sequencing technology.
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Several sequencing options have been developed and are available:
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Throughput: Up to 120 gigabases per run (400 million reads)
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Multiplexing: Ability to analyze a large number of libraries in a single run
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Read size: Up to 2x 300 bp
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Paired-ends: Sequencing inserts using forward and reverse primers
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Illumina MiSeq and Illumina NextSeq sequencing machines are available on site.
Sanger Sequencing​
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DNAVision is the leading DNA analysis company providing highest quality grade Sanger sequencing.
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DNAVision develops and validates sequencing projects in order to find, for example, new mutations in a gene of interest. In-house sequence analyses are used as the "gold standard" for the validation of genotyping assays.
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Sanger Sequencing is one of the most robust technique for clinical genotyping of SNP.
TaqMan Real-Time PCR Assays
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In combinaison with Sanger Sequencing, SNP genotyping using TaqMan assays is widely used in clinical projects for its robustness and accuracy.
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Each TaqMan SNP Genotyping Assay includes two allele-specific TaqMan MGB probes containing distinct fluorescent dyes to detect specific SNP targets.