SNPlex Genotyping System

The SNPlex® Genotyping System is a comprehensive solution for medium to large-scale genotyping studies based on capillary electrophoresis to separate selectively amplified gene regions. It is based on 12-48 multiplex SNP genotyping probes pools and insertion/deletion (up to 6 bases) and SNPs can be detected in the same assay.

The SNPlex system assay

The SNPlex system assay uses an oligonucleotide ligation assay (OLA) followed by PCR.  It can be divided into 3 basic steps:

1. Encoding-multiplex OLA to generate genotype-specific products
2. Amplification-multiplex PCR using a universal primer pair

* a. Hybridization of universal reporter probes to amplicons
* b. CE to rapidly identify eluted probes

Technical specifications

Applications

• Ideal for fine mapping, screening and QTL analysis projects
• Scalable multiplexing capability - from 12 - 48 SNPs/  ideal for projects of 30 to 1000s of SNPs
• Broad sample range - from 100s to 1000
• Choose your content or take advantage of the​ predefined SNPlex
• Linkage mapping set
• Design QC is available for a range of genomes including human, mouse, bovine, canine and 16 others