SNPlex Genotyping System

The SNPlex® Genotyping System is a comprehensive solution for medium to large-scale genotyping studies based on capillary electrophoresis to separate selectively amplified gene regions. It is based on 12-48 multiplex SNP genotyping probes pools and insertion/deletion (up to 6 bases) and SNPs can be detected in the same assay.

The SNPlex system assay

The SNPlex system assay uses an oligonucleotide ligation assay (OLA) followed by PCR.  It can be divided into 3 basic steps:

1. Encoding-multiplex OLA to generate genotype-specific products
2. Amplification-multiplex PCR using a universal primer pair

* a. Hybridization of universal reporter probes to amplicons
* b. CE to rapidly identify eluted probes

Technical specifications


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