RNALater Extraction

Since  microarrays entered  the diagnostic field, several protocols have been implemented to provide high quality RNA. One of the challenges was to fit with the routine of the surgeon and the anatomopathologist by preserving the RNA quality.

One of the most cost-effective solutions was to put the biopsies into dedicated solutions (RNAlater or equivalent) which preserve the RNA and allow  it to be transported at room temperature.

Description of services

Overview

Depending on  the requirements of our customers, we can extract  genomic DNA and  RNA (including  miRNAs) together or sequentially. This allows  several genomic applications  using the same sample without any sampling biases.

RNA extraction work flow:

• Tissue disruption using a polytron
• RNA extraction using either Trizol or a column-based method
• QC of the yield and the quality of extraction by NanoDrop or PicoGreen
• QC of the quality of RNA using an Agilent 2100 Bioanalyzer
• QC of the quality of DNA using an agarose gel

RNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric data (including the 260/280 and 260/230 ratios),the  RNA Integrity Number (RIN) values and the original electropherogram. The RNA can either be stored in our Biobank or shipped to your facility after the downstream process.

All samples prepared by DNAVision are routinely qualified with a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. For anything concerning the downstream application related to either next gen sequencing or microarray; an RIN value of minimum 7.5 is recommended.

DNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric data (including the 260/280 and 260/230 ratios),and a photograph of the agarose gel. The DNA can either be stored in our Biobank or shipped to your facility after the downstream process.

All DNA samples prepared by DNAVision are routinely qualified with a NanoDrop spectrophotometer or by PicoGreen and gel electrophoresis.

Some specific buffers are used to meet the requirements. of of  downstream applications like SNP genotyping , Sanger DNA Sequencing , Next Gen Sequencing or DNA Copy number analysis.

Turn-around time

Most projects are completed in one to two weeks. If you have a critical time requirement, we can often meet your needs.