DNAVision has extensive experience in providing nucleic acid extraction and purification services. We have expertise working with a wide range of sample types submitted from across the globe, with the proven ability to generate high yields of record quality RNA - RNAse free, free of PCR inhibitors and other contaminants.
DNAVision provides quality RNA extraction suitable for inclusion in regulatory submission projects. All work is conducted with stringent standard operating procedures under the direction of a client-dedicated project manager.
We routinely extract RNA from:
Brochure: Nucleic Acid Extraction at DNAVision
DNAVision routinely extract from most biological tissues/cells, including muscle, brain, bone etc. Reagents employed are mainly TRIzol reagents from Invitrogen, and column based technologies from Qiagen Inc.
On request from the customer an on-column DNase I digestion will be performed on previously extracted RNA. This procedure is performed in order to remove residual genomic DNA where this is critical to downstream processes (e.g. SAGE, Differential Display, some Q-PCR applications). RNeasy purification may also be performed on previously extracted RNA in order to remove residual proteins where this is critical to downstream processes.
RNA quantity is determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 nm wavelength. Each sample is confirmed for integrity using the Agilent BioAnalyzer and all results are returned as part of our Quality Control reporting process.
Following extraction and purification, the RNA is stored at -80°C. DNAVision offers to bank the samples for a period of one year free of charge, or for an extended period see BioBanking.
Our report includes the following elements : A260/A280 nm wavelength ratio, the RNA concentration, volume, total yield, and RIN, as well as 28S/18S ribosomal RNA ratio from the Bioanalyzer.
DNAVision routinely extracts RNA from PAXGENE tubes.
RNA yield is determined on a SpectraMAX 190 by measuring optical density at A260 nm wavelength.Each sample is confirmed for integrity using the Agilent BioAnalyzer and all results are returned as part of our Quality Control reporting process.
DNAVision guarantee a minimum yield of 3µg of RNA per PAXGENE tube (in >95% of the samples) as long as the tubes has been correctly filled with 2.5 ml of whole blood, and subsequently mixed thoroughly. This yield is sufficient for microarray analysis using DNAVision SOP's in which either 3 µg or 10µg RNA is needed for the labeling reaction after the globin reduction protocol and with a double or simple amplification.
Our report includes the A260/A280 nm wavelength ratio, the RNA concentration, volume, total yield, and RIN and 28S/18S ribosomal RNA ratio from the Bioanalyzer.
Following extraction and purification, RNA is stored at -80°C. DNAVision offers to bank the samples for a period of 1 year free of charge. For long term storage see BioBanking.
Reagents employed at DNAVision for RNA extraction from Formalin -fixed, Paraffin embedded Tissue is High Pure RNA Paraffin Kit from Roche. The yield of RNA is typically 1-1½ µg per cm2 of a 10µ tissue section. The main fraction of isolated RNA is in the size range of 150-250 bases.