RNA and DNA Extraction from Frozen Biopsies

Since the increase in  the use of  microarrays in molecular biology, a huge effort has been made to have high quality RNA and DNA. As RNA is highly sensitive to degradation (it  is less the case for DNA), most  researchers had to set-up "cold chains" for their sampling.

Description of services

Overview

Depending on  the requirements of our customers, we can extract  genomic DNA and  RNA (including  miRNAs) together or sequentially. This allows the  several genomic applications using the same sample without any sampling biases.

Extraction work flow:

• Tissue disruption using liquid nitrogen
• RNA extraction using either Trizol or column based methods
• QC of the yield and quality of extraction by NanoDrop or PicoGreen
• QC of the quality of RNA using an Agilent 2100 Bioanalyzer

• QC of the quality of DNA using an agarose gel

Slide preparation work flow:

In addition to  our core service for RNA extraction, we can also prepare some tissue sections  using a cryostat,  in order not to use up  the whole biopsy,  extracting only the RNA or DNA required for  further experiments.

Some of these sections can also be fixed and colored with  hematoxylin-eosin stain (or other, if required) and therefore analyzed for their cell contents. This protocol is very useful and even crucial in diagnostics to check the concordance between the RNA extracted  and the cells supposed to be analyzed.

RNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric data (including the 260/280 and 260/230 ratios), the RNA Integrity (RIN) values and the original electropherogram. This RNA can either be stored in our Biobank, or shipped to your facility after the downstream process.

All samples prepared by DNAVision are routinely qualified with a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. For anything  concerning the downstream application related to either next gen sequencing or microarray; an RIN value of minimum 7.5 is recommended

DNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric data (including the 260/280 and 260/230 ratios) and a  photograph of the agarose gel. The DNA can either be stored in our Biobank or shipped to your facility after the downstream process.

All DNA samples prepared by DNAVision are routinely qualified with a NanoDrop spectrophotometer or by PicoGreen and gel electrophoresis.

Some specific buffers are used to meet the requirements. of  downstream applications like SNP genotyping , Sanger DNA Sequencing , Next Gen Sequencing or DNA Copy number analysis,

Turn-around Time

Most projects are completed in one to two weeks. If you have a critical time requirement, we can often meet your needs.