DNA and RNA Extraction from Cell Lines

Eukaryotic or prokaryotic cells are widely used as models for biological studies and they can provide sufficient valuable  RNA and/or DNA for almost any application.

Description of Services

Overview

Depending on  the requirements of our customers, we can extract  the genomic DNA and RNA (including  miRNAs) either together or sequentially . This allows  several genomic applications using  the same sample without any sampling biases.Cell Lines

 

Work flow for sample preparation:

RNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric data (including the 260/280 and 260/230 ratios), the RNA Integrity Number (RIN) values and the original electropherogram. This RNA can either be stored in our Biobank or shipped to your facility after the downstream process.

All samples prepared by DNAVision are routinely qualified with a  NanoDrop spectrophotometer and an  Agilent 2100 Bioanalyzer. For anything concerning the downstream application related to either Next Gen Sequencinng or Microarray; an RIN value of minimum 7.5 is recommended.

DNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric data (including the 260/280 and 260/230 ratio) and photographs  of the agarose gel. The DNA can either be stored in our Biobank or shipped to your facility after the downstream process.

All DNA samples prepared by DNAVision are routinely qualified by NanoDrop or  PicoGreen and gel electrophoresis.

Depending on  downstream applications such as  SNP genotyping , Sanger DNA Sequencing , Next Gen Sequencing or DNA Copy number analysis some specific buffers are used..

Turnaround time

Most projects are completed in one to two weeks. If you have a critical time requirement, we can often meet your needs.

 

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