Exctraction of DNA and RNA from Blood

Blood and  other biofluids such as serum, plasma, urine and saliva are  high value non-invasive materials used in the discovery and development of biomarkers. They are also important for  future DNA/RNA  biomarker  molecular diagnostic tests.

Description of our services

Overview

Depending on  the requirements of our customers, we can extract  the genomic DNA and RNA (including  miRNAs) either together or sequentially . This allows  several genomic applications  using the same sample without any sampling biases.

Work flow for sample preparation:

• RNA extraction using a PAXgene Blood RNA Kit (Qiagen/Preanalytix) and DNA extraction using a column based method

N.B. We have also set up a new protocol if  miRNAs are required

• QC of the yield and  quality of  extraction by NanoDrop or PicoGreen
• QC of the quality of  RNA using an Agilent 2100 Bioanalyzer
• QC of the quality of  DNA using an agarose gel

RNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric  data (including the 260/280 and 260/230 ratios), the RNA Integrity Number (RIN) values and the original electropherogram. The RNA can either be stored in our Biobank or shipped to your facility after the downstream process.

All samples prepared by DNAVision are routinely qualified  with a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. For anything  concerning the downstream application related to either next gen sequencing or microarray; an RIN value of minimum 7.5 is recommended

DNA deliverables and QCs

For every extraction, we provide you with the spectrophotometric r data (including the 260/280 and 260/230 ratios) and a photographs of the agarose gel. The DNA can either be stored in our Biobank  or shipped to your facility after the downstream process.

All DNA samples prepared by DNAVision are routinely qualified with a NanoDrop spectrophotometer or by PicoGreen and gel electrophoresis.

Depending on  downstream applications such as like SNP genotyping , Sanger DNA Sequencing , Next Gen Sequencing or DNA Copy number analysis) some specific buffers are used.

Turnaround time

Most projects are completed in one to two weeks. If you have a critical time requirement, we can often meet your needs.