Quantitative PCR

The quantification of DNA and RNA is performed by quantitative PCR using fluorescence detection of the amplification products.
This technique is used in a wide range of applications in the context of the genetic characterization of biopharmaceuticals:

• qPCR:
  
  • Plasmid copy number
  • Residual DNA testing
  • Micro-organism contamination
• RT-QPCR:
  
  • Detection of the expression of a recombinant construction
  • Identification of phenotypic differentiation markers