Brochure: Next Gen Sequencing at DNAVision
DNAVision has extended its portfolio of high quality genomic services with Next Generation Sequencing using the Genome Sequencer FLX system (Roche). Through this breakthrough technology provided by DNAVision, a broad range of well known and new applications are available:
Titanium up-grade (allowing up to 400 Mb in a single run) and paired-end sequencing are already available.
Those applications are provided by DNAVision following our highest quality standards.
Currently, sequence data generation is no more a limiting factor. Data analysis and interpretation is now the key factor in Next Generation Sequencing projects. Thanks to its bioinformatic team, DNAVision is a real partner adding value to your research.
Performances and output of next generation sequencers (NGS) unravels those of classical sequencing, which nevertheless remains useful for specific applications alone or in combination with NGS.
DNAVision’s service with Roche Genome Sequencer FLX system
Feel free to contact us (info@dnavision.be) for any information on our next generation sequencing services, we will be pleased to find the most appropriate and effective technology for your research.
The whole genome sequence of virus, bacteria and fungi can be obtained in a single sequencing run. New SNPs, insertions/deletions can be rapidly identified through this whole genome sequencing.
This approach is particularly useful for new strain characterization, strain optimization, metabolic engineering or mutation discovery.
The genomic DNA is fragmented and a single stranded shotgun library is prepared before shotgun sequencing by GS FLX Titanium chemistry. This assay can generate up to 1 M of reads of 350-450 nt, allowing the simultaneous full genome sequencing of several bacteria/fungal strain in a single assay. Sequences are further assembled.
As the bioinformatic analysis is crucial for the project’s success, DNAVision also provides customized bio-informatic services (genome annotation, comparison with reference sequence ...) and complementary paired-end sequencing or gap-closing by primer walking. These services are fully optional and depend on your needs.
The objective of re-sequencing is to find correlation between sequence variation in exons or in specific DNA regions and particular phenotypes. It has a broad range of applications:
Re-sequencing of entire genome regions can be done starting from BAC libraries, capture-enriched samples or long-run PCR products. Smaller amplicon sequencing allows the ultra-deep sequencing of PCR product to analyze genetic variation within eukaryotic or prokaryotic populations. Specific regions of up to 192 individual samples can be simultaneously sequenced and compared using specifically designed Tag for sample pooling and small gaskets in the array.
Until recently, high throughput transcriptome studies were limited to well-characterized species with already EST or cDNA databases available. Now, the use of High Throughput Sequencing allows the full transcriptome profiling of any sample, whatever the studied species, even poorly characterized ones.
cDNA libraries can be massively sequenced by Hight Throughtput Sequencing. This approach is particularly useful to profile new or known EST and rare transcripts
After library preparation and clonal amplification by emulsion PCR (emPCR), the massive parallel sequencing is carried out with GS FLX Titanium reagents.
Depending on your needs, DNAVision provides customized bio-informatic services.
Metagenomics is the sequencing and analysis of DNA from environmental samples without the need for culturing individual, clonal organisms.
Until recently, metagenomics analysis was limited by the cost, low throughput and inherent cloning bias of the Sanger technologies. The Genome Sequencer FLX System provides a comprehensive view of metagenomics samples with high throughput, no cloning bias, and read lengths long enough to allow diversity and functional analysis of microbial communities like no other technology before. With more than one million reads per run, extensive survey can be conducted to get the whole picture of your sample.
Metagenomics samples can be sampled nearly everywhere, including several microenvironments within the human body, soil samples, extreme environments such as deep mines and the various layers within the ocean.
Metagenomic studies focus on specific hypervariable regions of the viral, bacterial or fungal genome to assess the species/genera diversity and composition within an environment. Using specifically developed multiplex identifier, the number of sequences per sample can be adapted to your specific project and budget. Metagenomics studies can also target the diversity of specific genes (coding for funtional enzymes …) within a microbial community.
As the bioinformatic analysis is crucial for the project’s success, DNAVision provides customized and optional QC and bio-informatic services.