DNA Sequencing

DNAVISION provides fast and quality sequencing

DNAVision SA offers a quick and high quality automated DNA sequencing analysis. We can offer sequencing service as research grade to GMP/GLP level.

Our DNA sequencing platform uses the speed of sequencers ( ABI PRISM 3730)with the latest generation of sequencing chemistry (BigDye V3.1).

Brochure: DNA Sequencing at DNAVision

Our Assets

Single or double strand sequencing
Plasmids, PCR products, EST, BAC end,…
Large insert : BAC, PAC, cosmid
High throughput DNA extraction
Rapid turnaround time

cGMP Sequencing

Quality for your submission

As specified by the FDA and cGMPs, rigorous standards must be adhered to in order to maintain a quality environment for performing contract services to support FDA/EMEA submissions.

FDA quality DNA sequencing:

Complete double-stranded coverage
Minimum 4-fold redundancy
100% accuracy Original electropherograms
Consensus sequence
Detailed sequencing strategy
Quality report
All project documentation archived for auditing purposes

Projects that are completed by DNAVision for submission are guaranteed to be 100% accurate with 100% double-stranded coverage of the sequenced region. Every base pair in the sequence region is resolved, even in areas of high GC content or difficult secondary structure.

High Throughput Sequencing

Sequencing for your research projects

DNAVision has extensive experience on large sequencing projects:

PCR products
Plasmids
EST and SAGE library
BAC / PAC and Cosmid end

96 Format Ready-to-Load Service capillary electrophoresis electronic data delivery up to 700 Bp read length 24 hour delivery time	Plasmid & PCR purified products
96 Format Speed Sequencing Service cycle sequencing reaction capillary electrophoresis electronic data delivery up to 700 Bp read length 3 day delivery time	Plasmid & PCR purified products

Genomic DNA Full Sequencing Service including DNA extraction/purification	Please inquire
Plasmid DNA Full Sequencing Service including plasmid DNA preparation & selection	Please inquire

Our capacities guarantee fast sample processing of even the largest samples.
All sequence data and chromatograms are available through our secure web interface.

"Ready-to-Load" Samples: DNAVision SA provides sequencing from "Ready-to-Load" samples. With this service, customers will do their own sequencing reactions and send us the cleaned and dried samples for sequencing. Usually the turnaround time for this service is one day.

Primer Walking

For your large DNA insert

The primer walking sequence allows for sequencing of a large DNA insert (1-20kb) with a very high speed (1.5 kb/week) and high precision (over 99.99%). This service includes design, synthesis of the primers, sequencing, and the assembly of the sequences. You will receive the edited consensus and all the sequence electropherograms obtained to cover the total insert.
DNAVision SA proposes three different primer walking strategies:

Single strand DNA sequencing*	Complete single-stranded coverage 1 Fold Redundancy 99% Accuracy Consensus sequence
Publication quality DNA sequencing*	Complete double-stranded coverage 2–3 Fold Redundancy 99.99% Accuracy Consensus sequence
FDA quality DNA sequencing*	Complete double-stranded coverage Minimum 4-Fold Redundancy 100% Accuracy Consensus sequence Detailed sequencing strategy Quality Report All project documentation archived for auditing purposes
All project documentation archived for auditing purposes

* Please inquire for pricing information.

Genomic Sequencing

You need large scale sequencing of prokaryotic or eukaryotic genomic DNA ?
DNAVision can provide you with this service.

* Please inquire for information.